Researchers from the Centre of Excellence in Molecular Biology, University of the Punjab, used CRISPR-Cas9 to knockdown vacuolar invertase (VInv) gene, which resulted to reduced cold-storage sugar formation and sweetening in potatoes. The findings are published in Planta.
CRISPR-Cas9-mediated knockdown of VInv gene was carried out using two sgRNAs in local cultivar of potato plants. The mRNA expression of VInv gene was reduced 90–99-fold in edited potato plants, in comparison with the non-edited potato plant. Chips analysis after cold storage led the researchers to the best potato lines. A five-fold reduction in the percentage of reducing sugars in tubers were found in one of the best lines, compared to the control. Physiological characteristics of the genome-edited potatoes were comparable with their conventional counterpart.
Yasmeen, A., Shakoor, S., Azam, S. et al. CRISPR/Cas-mediated knockdown of vacuolar invertase gene expression lowers the cold-induced sweetening in potatoes. Planta 256, 107 (2022).
Source: Crop Biotech Update