Genetic engineering tool generates antioxidant-rich purple rice

Researchers in China have developed a genetic engineering approach capable of delivering many genes at once and used it to make rice endosperm (seed tissue that provides nutrients to the developing plant embryo) produce high levels of antioxidant-boosting pigments called anthocyanins. The resulting purple endosperm rice holds potential for decreasing the risk of certain cancers, cardiovascular disease, diabetes, and other chronic disorders. The work was published recently in the journal Molecular Plant.

To date, genetic engineering approaches have been used to develop rice enriched in beta-carotene and folate, but not anthocyanins. Although these health-promoting compounds are naturally abundant in some black and red rice varieties, they are absent in polished rice grains because the husk, bran, and germ have been removed, leaving only the endosperm.

Previous attempts to engineer anthocyanin production in rice have failed because the underlying biosynthesis pathway is highly complex, and it has been difficult to efficiently transfer many genes into plants.

To address this challenge, Liu and his colleagues first set out to identify the genes required to engineer anthocyanin production in the rice endosperm. To do so, they analyzed sequences of anthocyanin pathway genes in different rice varieties.

Based on this analysis, they developed a transgene stacking strategy for expressing eight anthocyanin pathway genes specifically in the endosperm of the rice varieties. The resulting purple endosperm rice had high anthocyanin levels and antioxidant activity in the endosperm.

The researchers plan to evaluate the safety of purple endosperm rice as biofortified food, and they will also try to engineer the biosynthesis of anthocyanins in other crops to produce more purple endosperm cereals.

Molecular Plant, Zhu et al.: “Development of ”Purple Endosperm Rice” by Engineering Anthocyanin Biosynthesis in the Endosperm with a High-Efficiency Transgene Stacking System” 5 , DOI: 10.1016/j.molp.2017.05.008

Source: Рhys.org

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